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gia voeltz  (Addgene inc)


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    Structured Review

    Addgene inc gia voeltz
    Gia Voeltz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gia+voeltz/mCh-Drp1+(Plasmid+%2349152)/pm41507166-283-12-14
    Average 95 stars, based on 67 article reviews
    gia voeltz - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Construct:

    Article Title: Lipid droplets promote the aberrant liquid-liquid phase separation of alpha-synuclein leading to impaired energy homeostasis
    Article Snippet: .. The following constructs were used for transfections: mito-BFP was a gift from Gia Voeltz (Addgene plasmid # 49151 ; http://n2t.net/addgene:49151 ; RRID:Addgene_49151) ( Friedman et al , 2011 ). .. Lamp1-RFP was a gift from Walther Mothes (Addgene plasmid # 1817 ; http://n2t.net/addgene:1817 ; RRID:Addgene_1817) ( Sherer et al , 2003 ).

    Transfection:

    Article Title: Lipid droplets promote the aberrant liquid-liquid phase separation of alpha-synuclein leading to impaired energy homeostasis
    Article Snippet: .. The following constructs were used for transfections: mito-BFP was a gift from Gia Voeltz (Addgene plasmid # 49151 ; http://n2t.net/addgene:49151 ; RRID:Addgene_49151) ( Friedman et al , 2011 ). .. Lamp1-RFP was a gift from Walther Mothes (Addgene plasmid # 1817 ; http://n2t.net/addgene:1817 ; RRID:Addgene_1817) ( Sherer et al , 2003 ).

    Plasmid Preparation:

    Article Title: Lipid droplets promote the aberrant liquid-liquid phase separation of alpha-synuclein leading to impaired energy homeostasis
    Article Snippet: .. The following constructs were used for transfections: mito-BFP was a gift from Gia Voeltz (Addgene plasmid # 49151 ; http://n2t.net/addgene:49151 ; RRID:Addgene_49151) ( Friedman et al , 2011 ). .. Lamp1-RFP was a gift from Walther Mothes (Addgene plasmid # 1817 ; http://n2t.net/addgene:1817 ; RRID:Addgene_1817) ( Sherer et al , 2003 ).

    Article Title: Single-dye, transfection-free FLIM multiplexing via bioorthogonal chemistry
    Article Snippet: Culturing medium DMEM (12430054, 21063029 and A1443001), Opti-MEM with no phenol red (11058021) and FBS were all purchased from Thermo Fisher. .. The plasmid encoding Halo-TOMM20 was a gift from K. McGowan (HHMI Janelia, Halo-TOMM20-N-10, Addgene plasmid 123284); that encoding COX8A-SNAP was a gift from A. Egana (New England Biolabs, pSNAPf-COX8A, Addgene plasmid 101129); that encoding Halo-Sec61-C-18 was a gift from Kevin McGowan (Addgene plasmid # 123285; RRID:Addgene_123285); that encoding SNAP-Sec61β was a gift from Gia Voeltz (Addgene plasmid # 141152; RRID:Addgene_141152). .. HeLa cells (University of California, Berkeley, Cell Culture Facility) were cultured in DMEM (Thermo Fisher) supplemented with 10% FBS, penicillin (100 U mL −1 ) and streptomycin (100 μg mL −1 ).

    Article Title: Collagen fibril formation at the plasma membrane occurs independently from collagen secretion
    Article Snippet: .. BFP-KDEL was a gift from Gia Voeltz (Addgene plasmid # 49150) ( Friedman et al. , 2011 ). .. HP47-BFP-RDEL was generated using PCR products of HP47 (PCR amplified from plasmid MC202692 (Origene)) and BFP-RDEL (PCR amplified from BFP-KDEL plasmid) using the primers detailed in Extended data 16.

    Article Title: Regulation of lipoprotein processing by GPNMB in foamy macrophages: potential therapeutic targets for atherosclerosis
    Article Snippet: To generate a pEGFP-GPNMB fusion plasmid and a pEGFP-TFEC fusion plasmid, the ORF of mouse Gpnmb and human Tfec was subcloned into pEGFP-N3 (purchased from TSINGKE, China). mEmerald–RAB5A and mCherry–KDEL plasmids were gifts from Prof. Li Dong (National Laboratory of Biomacromolecules). σ2-mCherry was a gift from Stephen Royle (Addgene plasmid #186579; http://n2t.net/addgene:186579 ; RRID:Addgene_186579) . .. BFP-Sec61 beta was a gift from Gia Voeltz (Addgene plasmid #49154; http://n2t.net/addgene:49154 ; RRID:Addgene_49154) . mApple-Moesin-N-14 was a gift from Michael Davidson (Addgene plasmid #54928; http://n2t.net/addgene:54928 ; RRID:Addgene_54928). mPlum-Rab11a-7 was a gift from Michael Davidson (Addgene plasmid #55998; http://n2t.net/addgene:55998 ; RRID:Addgene_55998). mEos2-NEEP21-N-18 was a gift from Michael Davidson (Addgene plasmid #57407; http://n2t.net/addgene:57407 ; RRID:Addgene_57407). mCherry-MannII-N-10 was a gift from Michael Davidson (Addgene plasmid #55074; http://n2t.net/addgene:55074 ; RRID:Addgene_55074). ptdTomato-N1-FLCN was a gift from Shawn Ferguson (Addgene plasmid #49174; http://n2t.net/addgene:49174 ; RRID:Addgene_49174) . mCh-KIF5B*-strep was a gift from Juan Bonifacino (Addgene plasmid #120164; http://n2t.net/addgene:120164 ; RRID:Addgene_120164) . pCAG R2pH-LAMP1-3xFLAG was a gift from Massimiliano Stagi (Addgene plasmid #157940; http://n2t.net/addgene:157940 ; RRID:Addgene_157940) . pEGFP-N1-MITF-M (Addgene plasmid #38131; http://n2t.net/addgene:38131 ; RRID:Addgene_38131), pEGFP-N1-TFEB (Addgene plasmid #38119; http://n2t.net/addgene:38119 ; RRID:Addgene_38119), and pEGFP-N1-TFE3 (Addgene plasmid #38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120) were gifts from Shawn Ferguson . .. Monoclonal antibodies against mouse GPNMB (ab188222), GFP (ab183734), TFEC (ab185226), SEC22B (ab181076), ATP1A1 + ATP1A2 + ATP1A3 + ATP1A4 (ab300507), Rab5 (ab218624), LAMP1 (ab289548), and F4/80 (ab300421, Abcam) were purchased from Abcam.

    Article Title: Quantitative optical nanoscopy of mitochondrial-derived vesicles in neurons classifies pre-peroxisomal and clearing organelles
    Article Snippet: Neurons were transfected between DIV 5 and DIV 10 using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, 11668019), according to the instructions of the manufacturer. .. Plasmids used: OMP25-rsEGFP2 ; Halo-OMP25 ; SNAP-Sec-61β ; SNAP-OMP25 ; PEX16-rsEGFP2 ; mCherry-DRP1 was a gift from Gia Voeltz (Addgene plasmid # 49152; http://n2t.net/addgene:49152 ; RRID:Addgene_49152); mCherry-DRP1K38A was a gift from Samantha Lewis (Addgene plasmid # 232350; http://n2t.net/addgene:232350 ; RRID:Addgene_232350); Lamp1-RFP was a gift from Walther Mothes (Addgene plasmid # 1817; http://n2t.net/addgene:1817 ; RRID:Addgene_1817); PEX14-GFP was a kind gift from the Eggeling Lab. To generate SNAP-tagged TFAM and COX8A, the plasmid pAAV-EF1a-mCherry-IRES-WGA-Cre , a gift from Karl Deisseroth (Addgene plasmid #55632; http://n2t.net/addgene:55632 ; RRID:Addgene_55632), was digested with BamHI and EcoRI. ..

    Article Title: Collagen fibril formation at the plasma membrane occurs independently from collagen secretion
    Article Snippet: .. BFP-KDEL was a gift from Gia Voeltz (Addgene plasmid # 49150) (Friedman et al., 2011). .. HP47-BFP-RDEL was generated using PCR products of HP47 (PCR amplified from plasmid MC202692 (Origene)) and BFP-RDEL (PCR amplified from BFP-KDEL plasmid) using the primers detailed in Extended data 16.

    Article Title: Regulation of lipoprotein processing by GPNMB in foamy macrophages: potential therapeutic targets for atherosclerosis.
    Article Snippet: To generate a pEGFP-GPNMB fusion plasmid and a pEGFP-TFEC fusion plasmid, theORFofmouseGpnmb and humanTfecwas subcloned into pEGFP-N3 (purchased from TSINGKE, China). mEmerald–RAB5A and mCherry–KDEL plasmids were gifts from Prof. Li Dong (National Laboratory of Biomacromolecules). σ2-mCherry was a gift from Stephen Royle (Addgene plasmid #186579; http://n2t.net/addgene: 186579; RRID:Addgene_186579)67. .. BFP-Sec61 beta was a gift from Gia Voeltz (Addgene plasmid #49154; http://n2t.net/addgene:49154; RRID:Addgene_49154)68. mApple-Moesin-N-14 was a gift from Michael Davidson (Addgene plasmid #54928; http://n2t.net/addgene:54928; RRID:Addgene_54928). mPlum-Rab11a-7 was a gift from Michael Davidson (Addgene plasmid #55998; http://n2t.net/addgene:55998; RRID:Addgene_55998). mEos2-NEEP21-N-18 was a gift from Michael Davidson (Addgene plasmid #57407; http://n2t.net/addgene:57407; RRID:Addgene_57407). mCherry-MannII-N-10 was a gift from Michael Davidson (Addgene plasmid #55074; http://n2t.net/addgene:55074; RRID:Addgene_55074). ptdTomato-N1-FLCN was a gift from Shawn Ferguson (Addgene plasmid #49174; http://n2t.net/addgene:49174; RRID:Addgene_49174)69. mCh-KIF5B*-strep was a gift from Juan Bonifacino (Addgene plasmid #120164; http://n2t.net/addgene:120164; RRID:Addgene_120164)70. pCAG R2pH-LAMP1-3xFLAG was a gift from Massimiliano Stagi (Addgene plasmid #157940; http://n2t.net/ addgene:157940; RRID:Addgene_157940)71. pEGFP-N1-MITF-M (Addgene plasmid #38131; http://n2t.net/addgene:38131; RRID:Addgene_38131), pEGFP-N1-TFEB (Addgene plasmid #38119; http://n2t.net/addgene:38119; RRID:Addgene_38119), and pEGFP-N1TFE3 (Addgene plasmid #38120; http://n2t.net/addgene:38120; RRID:Addgene_38120) were gifts from Shawn Ferguson72. .. Monoclonal antibodies against mouse GPNMB (ab188222), GFP (ab183734), TFEC (ab185226), SEC22B (ab181076), ATP1A1 + ATP1A2 + ATP1A3 +ATP1A4 (ab300507), Rab5 (ab218624), LAMP1 (ab289548), and F4/80 (ab300421, Abcam) were purchased from Abcam.

    other:

    Article Title: Quantitative optical nanoscopy of mitochondrial-derived vesicles in neurons classifies pre-peroxisomal and clearing organelles.
    Article Snippet: Neuronal transfection and live-cell staining AR TI CL E IN P RE SS ARTICLE IN PRESS Neurons were transfected between DIV5 and DIV10 using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, 11668019), according to the instructions of the manufacturer.



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    Addgene inc gia voeltz
    Gia Voeltz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gia+voeltz/mCh-Drp1+(Plasmid+%2349152)/pm41507166-283-12-14
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